BACTERIAL ISOLATES FROM PEDIATRIC BLOOD CULTURE AND THEIR RESPONSE TO ANTIMICROBIAL AGENTS

Authors

  • Sherko A Omer Department of Microbiology, School of Medicine, Faculty of Medical Sciences, University of Sulaimani, Kurdistan Region, Iraq.

DOI:

https://doi.org/10.17656/jsmc.10067

Keywords:

Pediatric blood culture, Bacteremia, Sulaimani, Antimicrobial susceptibility

Abstract

Background

The blood stream can be invaded by many bacteria in the course of many infections resulting in bacteremia or septicemia.

Objectives

The aim of this study was to identify the frequency and the types of bacteria isolated form pediatric blood cultures and identify their susceptibility to antimicrobial agents.

Patients and Methods

A retrospective study on the laboratory reports of blood cultures from pediatric patients attended Sulaimani Pediatric Teaching Hospital during 18 months period.

Results

From 6024 processed blood cultures, 512 cultures yielded positive for growth of bacteria (8.4%). Staphylococcus aureus was the commonest microorganism isolated from both Neonatal Intensive Care Unit (NICU) and other hospital units. This was followed by Enterobacter spp. and Escherichia coli in NICU, while in other hospital units Staphylococcus aureus was followed by E. coli and Enterobacter spp.. Salmonella Typhi was isolated from 5.8% of blood cultures. The isolates mostly were resistant to many antimicrobial drugs. Certain antimicrobial agents showed to be more effective such as vancomycin, amikacin and cefaclor while more than 80% of the isolates were resistant to other agents such as ampicillin, oxacillin, carbincillin and ceftriaxone.

Conclusion

Staphylococcus aureus was the commonest bacteria isolated from blood cultures followed by Gram negative Enterobacteriaceae. The isolated bacteria were resistant to many antimicrobial agents while vancomycin andamikacin were found to be more effective than other agents.

References

Seifert H, Wisplinghoff H. Bloodstream infection and endocarditis. In: S. Peter Borriello, . PRM, Funke G, editors. Topley and Wilson’s Microbiology and Microbial Infections. 1. 10 Edition ed. UK: Wiley; 2005. p. 1-46.

Darby JM, Linden P, Pasculle W, Saul M. Utilization and diagnostic yield of blood cultures in a surgical intensive care unit. Critical care medicine. 1997 Jun;25(6):989-94. PubMed PMID: 9201052. DOI: https://doi.org/10.1097/00003246-199706000-00016

Roberts FJ, Geere IW, Coldman A. A three-year study of positive blood cultures, with emphasis on prognosis. Reviews of infectious diseases. 1991 Jan-Feb;13(1):34-46. PubMed PMID: 2017629. DOI: https://doi.org/10.1093/clinids/13.1.34

Weinstein MP, Murphy JR, Reller LB, Lichtenstein KA. The clinical significance of positive blood cultures: a comprehensive analysis of 500 episodes of bacteremia and fungemia in adults. II. Clinical observations, with special reference to factors influencing prognosis. Reviews of infectious diseases. 1983 Jan-Feb;5(1):54-70. PubMed PMID: 6828812. DOI: https://doi.org/10.1093/clinids/5.1.54

Clark R, Powers R, White R, Bloom B, Sanchez P, Benjamin DK, Jr. Nosocomial infection in the NICU: a medical complication or unavoidable problem? Journal of perinatology : official journal of the California Perinatal Association. 2004 Jun;24(6):382-8. PubMed PMID: 15116140. DOI: https://doi.org/10.1038/sj.jp.7211120

Brady MT. Health care-associated infections in the neonatal intensive care unit. American journal of infection control. 2005 Jun;33(5):268-75. PubMed PMID: 15947743. DOI: https://doi.org/10.1016/j.ajic.2004.11.006

Stolfi I MM, Lana S (1999) . 25:193–200. Frequenza e variabilità delle infezioni ospedaliere in Terapia Intensiva Neonatale (TIN). Riv Ital Pediatr. 1999;25:193-200. DOI: https://doi.org/10.1353/esc.1999.0006

Stoll BJ, Hansen N, Fanaroff AA, Wright LL, Carlo WA, Ehrenkranz RA, et al. Late-onset sepsis in very low birth weight neonates: the experience of the NICHD Neonatal Research Network. Pediatrics. 2002 Aug;110(2 Pt 1):285-91. PubMed PMID: 12165580. DOI: https://doi.org/10.1542/peds.110.2.285

Watson RS, Carcillo JA, Linde-Zwirble WT, Clermont G, Lidicker J, Angus DC. The epidemiology of severe sepsis in children in the United States. American journal of respiratory and critical care medicine. 2003 Mar 1;167(5):695-701. PubMed PMID: 12433670. DOI: https://doi.org/10.1164/rccm.200207-682OC

Herz AM, Greenhow TL, Alcantara J, Hansen J, Baxter RP, Black SB, et al. Changing epidemiology of outpatient bacteremia in 3- to 36-month-old children after the introduction of the heptavalent-conjugated pneumococcal vaccine. The Pediatric infectious disease journal. 2006 Apr;25(4):293-300. PubMed PMID: 16567979. DOI: https://doi.org/10.1097/01.inf.0000207485.39112.bf

Patrick CC. Coagulase-negative staphylococci: pathogens with increasing clinical significance. The Journal of pediatrics. 1990 Apr;116(4):497-507. PubMed PMID: 2181097. DOI: https://doi.org/10.1016/S0022-3476(05)81593-8

Albano EA, Pizzo PA. Infectious complications in childhood acute leukemias. Pediatric clinics of North America. 1988 Aug;35(4):873-901. PubMed PMID: 3047658. DOI: https://doi.org/10.1016/S0031-3955(16)36514-2

Centers for Disease C. Increase in National Hospital Discharge Survey rates for septicemia--United States, 1979-1987. MMWR Morbidity and mortality weekly report. 1990 Jan 19;39(2):31-4. PubMed PMID: 2104656.

Mishra UK, Jacobs SE, Doyle LW, Garland SM. Newer approaches to the diagnosis of early onset neonatal sepsis. Archives of disease in childhood Fetal and neonatal edition. 2006 May;91(3):F208-12. PubMed PMID: 16632649. Pubmed Central PMCID: 2672708. DOI: https://doi.org/10.1136/adc.2004.064188

Stronati M, Borghesi A. Neonatal Bacterial and Fungal Infections. In: Buonocore G, Bracci R, Weindling M, editors. Neonatology A Practical Approach to Neonatal Diseases. Italy: Springer; 2012. p. 905-30. DOI: https://doi.org/10.1007/978-88-470-1405-3_116

Paisley JW, Lauer BA. Pediatric blood cultures. Clinics in laboratory medicine. 1994 Mar;14(1):17-30. PubMed PMID: 8181229. Epub 1994/03/01. eng. DOI: https://doi.org/10.1016/S0272-2712(18)30391-3

Shoji K, Komuro H, Watanabe Y, Miyairi I. The utility of anaerobic blood culture in detecting facultative anaerobic bacteremia in children. Diagnostic microbiology and infectious disease. 2013 Aug;76(4):409-12. PubMed PMID: 23747032. Epub 2013/06/12. eng. DOI: https://doi.org/10.1016/j.diagmicrobio.2013.05.003

York MK. Blood Cultures- General Detction and Interpretation. In: Isenburg HD, editor. Clinical Microbiology Procedures Handbook. 1. 2 ed. Washington, D.C.: American Society for Microbiology; 2007. p. 3.4.1.-3.4.1.19.

Weinstein MP. Current blood culture methods and systems: clinical concepts, technology, and interpretation of results. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America. 1996 Jul;23(1):40-6. PubMed PMID: 8816127. DOI: https://doi.org/10.1093/clinids/23.1.40

Colaninno PM. Identification of Gram-Positive Organisms. In: Goldman E, H. GL, editors. Practical Handbook of Microbiology. Second ed. Boca Raton CRC Press; 2009. p. 53-66. DOI: https://doi.org/10.1201/9781420009330.ch5

Donna J. Kohlerschmidt, Kimberlee A. Musser, Dumas NB. Identification of Gram-Positive Organisms In: Goldman E, H. GL, editors. Practical Handbook of Microbiology. Boca Raton: CRC Press; 2009. p. 67-79.

Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr;45(4):493-6. PubMed PMID: 5325707. DOI: https://doi.org/10.1093/ajcp/45.4_ts.493

CLSI. Performance Standards for Antimicrobial Disk Susceptibility Tests: Twenty-First Informational Supplement. Wayne, PA2011.

Bryan CS. Clinical implications of positive blood cultures. Clinical microbiology reviews. 1989 Oct;2(4):329-53. PubMed PMID: 2680055. Pubmed Central PMCID: 358128. DOI: https://doi.org/10.1128/CMR.2.4.329

Kaplan SL, Vallejo JG. Bacteremia and Septic Shock. 2009. In: Feigin and Cherry’s Textbook of Pediatric Infectious Diseases [Internet]. USA: ELSEVIER; [837-51]. DOI: https://doi.org/10.1016/B978-1-4160-4044-6.50074-1

Hall KK, Lyman JA. Updated review of blood culture contamination. Clinical microbiology reviews. 2006 Oct;19(4):788-802. PubMed PMID: 17041144. Pubmed Central PMCID: 1592696. Epub 2006/10/17. eng. DOI: https://doi.org/10.1128/CMR.00062-05

Stalnikowicz R, Block C. The yield of blood cultures in a department of emergency medicine. European journal of emergency medicine : official journal of the European Society for Emergency Medicine. 2001 Jun;8(2):93-7. PubMed PMID: 11436922. DOI: https://doi.org/10.1097/00063110-200106000-00004

Stoesser N, Moore CE, Pocock JM, An KP, Emary K, Carter M, et al. Pediatric bloodstream infections in Cambodia, 2007 to 2011. The Pediatric infectious disease journal. 2013 Jul;32(7):e272-6. PubMed PMID: 23838788. Epub 2013/07/11. eng. DOI: https://doi.org/10.1097/INF.0b013e31828ba7c6

Wisplinghoff H, Seifert H, Tallent SM, Bischoff T, Wenzel RP, Edmond MB. Nosocomial bloodstream infections in pediatric patients in United States hospitals: epidemiology, clinical features and susceptibilities. The Pediatric infectious disease journal. 2003 Aug;22(8):686-91. PubMed PMID: 12913767. Epub 2003/08/13. eng. DOI: https://doi.org/10.1097/01.inf.0000078159.53132.40

Lee CY, Chen PY, Huang FL, Lin CF. Microbiologic spectrum and susceptibility pattern of clinical isolates from the pediatric intensive care unit in a single medical center - 6 years’ experience. Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi. 2009 Apr;42(2):160-5. PubMed PMID: 19597649. Epub 2009/07/15. eng.

Babay HA, Twum-Danso K, Kambal AM, Al-Otaibi FE. Bloodstream infections in pediatric patients. Saudi medical journal. 2005 Oct;26(10):1555-61. PubMed PMID: 16228055.

Cordero L, Rau R, Taylor D, Ayers LW. Enteric gram-negative bacilli bloodstream infections: 17 years’ experience in a neonatal intensive care unit. American journal of infection control. 2004 Jun;32(4):189-95. PubMed PMID: 15175611. Epub 2004/06/04. eng. DOI: https://doi.org/10.1016/j.ajic.2003.07.004

Blomberg B, Jureen R, Manji KP, Tamim BS, Mwakagile DS, Urassa WK, et al. High rate of fatal cases of pediatric septicemia caused by gram-negative bacteria with extended-spectrum beta-lactamases in Dar es Salaam, Tanzania. Journal of clinical microbiology. 2005 Feb;43(2):745-9. PubMed PMID: 15695674. Pubmed Central PMCID: 548071. Epub 2005/02/08. eng. DOI: https://doi.org/10.1128/JCM.43.2.745-749.2005

Kruse AY, Thieu Chuong DH, Phuong CN, Duc T, Graff Stensballe L, Prag J, et al. Neonatal bloodstream infections in a pediatric hospital in Vietnam: a cohort study. Journal of tropical pediatrics. 2013 Jul 18. PubMed PMID: 23868576. Epub 2013/07/23. Eng.

Mohammad MS, Omer SA, Radhid BR, Hamawandi AM. Enteric Fever in Sulaimani Pediatric Teaching Hospital: Risk factors, Presentation, and Drug Susceptibility. Journal of Sulaimani Medical College 2014;4(1):55-62. DOI: https://doi.org/10.17656/jsmc.10049

Hsueh PR, Chen WH, Luh KT. Relationships between antimicrobial use and antimicrobial resistance in Gram-negative bacteria causing nosocomial infections from 1991-2003 at a university hospital in Taiwan. International journal of antimicrobial agents. 2005 Dec;26(6):463-72. PubMed PMID: 16280243. Epub 2005/11/11. eng. DOI: https://doi.org/10.1016/j.ijantimicag.2005.08.016

Al-Rawazq HS, Mohammed AK, Al-Zubaidy RH. Bacterial Isolates in Blood Culture of Children with Septicemia. Fac Med Baghdad. 2012;54(1):96-9. DOI: https://doi.org/10.32007/jfacmedbagdad.541781

Jumah DS, Hassan MaK. Predictors of Mortality Outcome in Neonatal Sepsis. The Medical Journal Basrah University. 2007;25, No.1, 2007(1):11-8. DOI: https://doi.org/10.33762/mjbu.2007.48118

Abass VT, Omer SA. Oral Findings and Microflora in Type II Diabetes Mellitus in Sulaimani City. Journal of Sulaimani Medical College. 2011;1(1):13-28. DOI: https://doi.org/10.17656/jsmc.10011

Fathi NN, Omer SA, Mohammed MO. Nasal Carriers of Methicillin-Resistant Staphylococcus aureus (MRSA), Infection Safety and Hand Hygiene Among Health Workers in Sulaimani City. Journal of Sulaimani Medical College. 2013;3(1):1-12. DOI: https://doi.org/10.17656/jsmc.10026

Karwan Raoof Hassan IHAaZSR. Types of Organisms Causing Urinary Tract Infection and Their Antibiotic Sensitivity Pattern in Sulaimani Teaching Hospital. Journal of Sulaimani Medical College. 2014;4(2):11-7. DOI: https://doi.org/10.17656/jsmc.10044

Shafazand S, Weinacker AB. Blood cultures in the critical care unit: improving utilization and yield. Chest. 2002 Nov;122(5):1727-36. PubMed PMID: 12426278. DOI: https://doi.org/10.1378/chest.122.5.1727

Jones RN. Resistance patterns among nosocomial pathogens: trends over the past few years. Chest. 2001 Feb;119(2 Suppl):397S-404S. PubMed PMID: 11171776. Epub 2001/02/15. eng. DOI: https://doi.org/10.1378/chest.119.2_suppl.397S

Published

2015-06-01

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How to Cite

1.
Omer S. BACTERIAL ISOLATES FROM PEDIATRIC BLOOD CULTURE AND THEIR RESPONSE TO ANTIMICROBIAL AGENTS. JSMC [Internet]. 2015 Jun. 1 [cited 2024 Jul. 25];5(1):51-6. Available from: https://jsmc.univsul.edu.iq/index.php/jsmc/article/view/jsmc-10067

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