EVALUATION OF SECRETED FRIZZLED-RELATED PROTEIN-2 METHYLATION STATUS IN PATIENTS WITH CHRONIC MYELOID LEUKEMIA
Volume 13 , Issue 3 , September 2023
Authors
Mohammed Hassan Tahir 1 ; Dlnya Asad Mohamad ; Mohammad Hamid 2
1 Department of Biology, University of Sulaimani, Kurdistan Region, Iraq.
2 Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
10.17656/jsmc.10418
Keywords
Abstract
Background
Chronic Myeloid Leukemia (CML) is a myeloproliferative disorder defined by the chromosomal translocation
(t) (9;22) encoding for the BCR-ABL fusion gene. Tumor development and progression may be affected by
hypermethylation of promoter regions of certain genes. Secreted Frizzled-Related Proteins (SFRPs) genes
produce Wnt signaling antagonists, and their epigenetic silencing has been detected in multiple types of
leukaemia. While epigenetic silencing has been broadly reported for SFRPs, SFRP2 is a family member less
studied in leukaemia.
Objectives
To evaluate SFRP2 gene methylation patterns in CML patients compared with healthy individuals.
Materials and Methods
Sodium bisulfite was applied to the extracted DNA from blood samples of 75 CML patients and 25 healthy
subjects who served as the control group. The DNA was then analyzed by methylation-specific high-resolution
melting (MS-HRM) using specific primers for the SFRP2 gene in promoter sequence.
Results
Forty-five out of 75 CML patients investigated were shown to have SFRP2 methylation percentages between
50% to 100%, whereas 19 out of 25 healthy subjects had a methylation level of less than 50%.
Conclusion
The current study demonstrated that SFRP2 promoter hypermethylation exists in CML, as in several other
solid tumours. Hence, methylation of this gene may play a part in initiating leukemogenesis
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- Published at21 September 2023
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