DETECTION OF KRAS MUTATIONS AMONG LOCAL PANCREATIC CANCER PATIENTS

Volume 14 , Issue 2 , June 2024 , Pages 239-250

Authors

Sadoun Ezzat Abdoullah 1 ; Karzan Ghafur Khidhir 1

1 Department of Biology, College of Science, University of Sulaimani, Kurdistan Region, Iraq.

DOI logo 10.17656/jsmc.10471

Keywords

Abstract


Background
Pancreatic cancer poses a significant oncological challenge in the field of human health due to its aggressive
behaviour and restricted scope of available treatment choices. Its multifaceted origin combined with genetic,
environmental, and lifestyle factors, makes identification and management of this condition difficult.
Objectives
This study aims to investigate major KRAS gene mutations among local pancreatic cancer patients and further
unveil underlying molecular mechanisms involved in the pathogenesis of this malignancy.
Materials and Methods
Blood and solid tissue samples were collected from patients diagnosed with pancreatic cancer after obtaining
appropriate ethical approval. Genomic DNA was extracted, quality and quantity were evaluated prior to
conducting gene mutation analysis using allele-specific PCR and specific primers for the detection of KRAS
gene mutations in human tissues.
Results
Our data indicates that localization of tumors in the head of the pancreas was the most common among
our study population; 34.5% of the patients were overweight according to body mass index; 21.9% had a
history of other cancers, while 37.5% had a family history of pancreatic cancer. The KRAS mutation analysis
detected 7 KRAS mutations in pancreatic tumor tissue: KRASG12C, KRASG12V, KRASG12D, KRASG13D,
KRASG12R, KRASG12S, KRASG12A. The KRASG12C had the highest mutation rate (81.3%), followed by
KRASG12V (73.5.4%) in pancreatic cancer patients.
Conclusion
Our findings highlight the importance of further investigating KRASG12C and KRASG12V mutations in the
KRAS gene which may have implications for finding better diagnostic and targeted treatment strategies.

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  • Published at21 June 2024

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