MOLECULAR TYPING OF MRSA ISOLATED FROM SULAIMANIYAH CITY HOSPITAL USING DIFFERENT MOLECULAR TECHNIQUES

Volume 14 , Issue 1 , March 2024 , Pages 73-87

Authors

Poshya Omer Hama 1 ; Sahand Kamaluldeen Arif 1 ; Huner Hiwa Arif 1

1 Department of Biology, College of Science, University of Sulaimani, Kurdistan Region, Iraq.

DOI logo 10.17656/jsmc.10453

Keywords

Abstract

Background
Staphylococcus aureus causes a variety of human illnesses, methicillin-resistant S. aureus is the deadliest and
most dangerous in clinical settings.
Objectives
Genotype detection of MRSA using Staphylococcal Cassette vChromosome mec (SCCmec) and Enterobacterial
Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) techniques.
Methods
Fifty-two isolates were taken from Burn-and-Plastic Surgery Hospital/Emergency. The samples were collected
from different sources from July to December 2021. All samples were cultivated and identifi ed as S.aureus
with coa and nuc genes. Cefoxitin disk, mecA, and mecC genes were used for MRSA detection. Three sets of
SCCmec typing and ERIC PCR were used for genotypic detection, in addition, the ability to form biofi lm was
investigated.
Results
Forty-seven isolates out of 52 (90.38%) and 50/52 (96.15%) were positive for nuc and coa gene, respectively.
Twenty-one out of 52 isolates (40.38%) were resistant to cefoxitin and positive for mecA gene while all of them
were negative for the mecC gene. Seven SCCmec types were found, type III was the most predominant 9/21
(42.8%) then types II 5/21 (23.8%), V 2/21 (9.5%), VI 2/21 (9.5%), I 1/21 (4.7%), IV 1/21 (4.7 %), and type VIII
1/21 (4.7%) respectively. There was no detection for Set III, of fi fteen genotyped MRSA used in the ERIC
PCR, three clusters showed (C1, C2, C3) among them 40% (6/15) showed high genetic diversity this suggests
that these subgroups might have shared a common ancestor. Biofi lm formation showed 11/21 (50%) strong
produces, 7/21 (33.3%) moderate produces and 3/21 (14.2%) weak producers.
Conclusion
Type VI and VIII were discovered for the fi rst time in this area. The ERIC-PCR fi ndings revealed that 60%
of the isolates were the same and distributed in three separate groups, which suggests a dissemination of
bacterial infection among the patients belonging to the groups.

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  • Published at21 March 2024

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