Nabil AM Salmo *, Azad Kareem Saeed **, Snur Muhammad-Amin Hassan *** and Hasanaein H Khudeir *

*   Department of Pathology and Forensic Pathology, School of Medicine, Sulaimani University.

** Department of Histopathology, College of Veterinary Medicine, Sulaimani University.

Submitted: 17/9/2013; Accepted: 18/2/2014; Published 1/12/2014

DOI Link: https://doi.org/10.17656/jsmc.10056  



Exposure to ultraviolet type B (UVB) radiation induces a number of pathologic changes in skin, including erythema, edema, epidermal hyperplasia, sunburn cell formation, immune suppression and eventually leads to cancer development.


To elucidate the differences in histological appearances of mast cells and apoptotic bodies between the two species (mice and human) among hyperkeratotic and acanthotic types of seborrheic keratosis (SK).

Materials and Methods

Thirty paraffin blocks were used in this study;  fifteen histologically con rmed acanthotic and hyperkeratotic SK cases in human (9 acanthotic and 6 hyperkeratotic) and  fifteen blocks from both types acanthotic and hyperkeratotic SK cases in mice induced by UVB light (9 acanthotic and 6 hyperkeratotic).


Our results revealed that there was a signi cant correlation between mast cells and apoptotic bodies for both groups according to Pearson Correlation test. In human cases mast cells counting ranged between 2-10 with a mean number of 5.2/1HPF, while the total number of apoptotic bodies ranged from 1-4 with a mean number of 2.6/10HPF. When compared to mice cases, the number of mast cells were increased with a range of 12-23 and with a mean number of 19.067/1HPF, while apoptotic bodies were decreased with a range of 3-20/10HPF and with a mean number of 9.4/10HPF.


Dermal mast cells in ltration were remarkably increased in mice skin specimens which were exposed to UVB. The number of apoptotic bodies in UVB induced cases were more than in human sporadic cases.


UVB, Seborrheic keratosis, Apoptotic bodies, Mast cells.


1. Naruke Y, Nakashima M, Suzuki K, Matsuu-Matsuyama M, Shichijo K, Kondo H, et al. Alteration of p53-binding protein 1 expression during skin carcinogenesis: Association with genomic instability. Cancer Science. 2008; 99: 946-51.

2. Saeed AK, Salmo N. Epidermal growth factor receptor expression in mice skin upon ultraviolet B exposure-seborrheic keratosis as a coincidental and unique finding. Adv Biomed Res. 2012; 1: 59.

3. Bhatia N, Demmer TA, Sharma AK, Elcheva I, Spiegelman VS. Role of β-TrCP ubiquitin ligase receptor in UVB mediated responses in skin. Arch Biochem Biophys. 2011; 508: 178-84.

4. Banerjee S. Seborrhoeic keratosis: Bilaterally symmetrical linear verrucous lesions in inguinal folds, an unusual presentation. Journal of Pakistan Association of Dermatologists. 2012; 22: 73-5.

5. Hafner C, Hartmann A, Van Oers A MM, Stoehr R, Zwarthoff EC, Hofstaedter F, et al. FGFR3 mutations in seborrheic keratoses are already present in flat lesions and associated with age and localization. Mod Pathol. 2007; 20: 895-903.

6. Ming M, Shea CR, Feng L, Soltani K, He YY. UVA Induces lesions resembling seborrheic keratoses in mice with keratinocyte-specific PTEN down-regulation. J Invest Dermatol. 2011; 131: 1583-6.

7. Lee ES, Whang MR and Kang WH. Absence of human papillomavirus DNA in nongenital seborrheic keratosis. J Korean Med Sci. 2001; 16: 619-22.

8. Bhuiyan ZH. Seborrheic keratosis: A case report. The Orion Medical Journal. 2007; 26: 441-2.

9. Storm CA, Elder DE. The skin. In: Rubin E, Reisner HM. Essentials of Rubin’s Pathology. 5th ed. Philadelphia: Lippincott Williams & Wilkins; 2009.pp518.

10. Storm CA, Elder DE. The skin. In: Rubin R and Strayer DS. Rubin’s Pathology: Clinicopathologic Foundations of Medicine. 5th ed. Baltimore: Lippincott Williams & Wilkins; 2008.pp1049. 

11. Kwon OS, Hwang EJ, Bae JH. Seborrheic keratosis in the Korean males: Causative role of sunlight. Photodermatol Photoimmunol Photomed. 2003; 19: 73-80.

12. Wang JF, Wang B, Shehan JM, Sarma DP. Acantholytic Seborrheic Keratosis. The Internet Journal of Dermatology. 2008; 6: 1-4.

13. Kirkham N. Tumors and cysts of the epidermis. In: Elder DE, Elenitsas R, Johnson B, Murphy GF, Xu X. Lever’s Histopathology of the Skin, 10th ed. India: Lippincott Williams &Wilikins; 2009. pp795-8

13. Murphy GF, Sellheyer K, Mihm MC. The skin. In: Kumar V, Abbas AK, Favsto N. Skin neoplasm. Robbins and Cotran Pathologic Basis of Disease. 7th ed. China: Elsevier Saunders; 2005. pp1230.

14. Rajabi P, Adibi N, Nematollahi P, Heidarpour M, Eftekhari M, Siadat AH. Bowenoid transformation in seborrheic keratosis: A retrospective analysis of 429 patients. J Res Med Sci. 2012; 17: 217-21.

15. Logie A, Dunois-Larde C, Rosty C. Activating mutations of the tyrosine kinase receptor FGFR3 are associated with benign skin tumors in mice and humans. Hum Mol Genet. 2005; 14: 1153-60.

13. Hassan SMA. The role of Acetyl salicylic acid on COX-2 expression in UVB- irradiated skin mouse utilizing immunohistochemistry. MSc thesis: University of Sulaimani; Iraq, 2011.

17. Kulms D, Schwarz T. Molecular mechanisms of UV-induced apoptosis. Photodermatology, Photoimmunology & Photomedicine. 2000; 16: 195-201.

18. Claerhout S, Laethem AV, Agostinis P, Garmyn M. Pathways involved in sunburn cell formation: Deregulation in skin cancer. Photochem Photobiol Sci. 2006; 5: 199-207.

19. Katiyar SK, Mantena SK, Meeran SM. Silymarin protects epidermal keratinocytes from ultraviolet radiation-induced apoptosis and DNA damage by nucleotide excision repair mechanism. Plos One. 2011; 6: 1-11.

20. Byrne SN, Limo´n-Flores AY, Ullrich SE. Mast cell migration from the skin to the draining lymph nodes upon ultraviolet irradiation represents a key step in the induction of immune suppression. J Immunol. 2008; 180: 4648-55.

21. Huang B, Lei Z, Zhang G, Dong L, Song C, Li B, et al. SCF-mediated mast cell infiltration and activation exacerbate the inflammation and immunosuppression in tumor microenvironment. Blood. 2008; 112: 1269-79.

23. Balin AK. Seborrheic keratosis, http://emedicine.medscape.com/article/1059477-overview, retrieved 2013.

24. Bowen AR, Hanks AN, Murphy KJ, Florell SR, Grossman D. Proliferation, apoptosis and survivin expression in keratinocytic neoplasms and hyperplasia. Am J Dermatopathol. 2004; 26: 177-81.

25. Kligman LH, Murphy GF. Ultraviolet B radiation increases hairless mouse mast cells in a dose-dependent manner and alters distribution of UV-induced mast cell growth factor. Photochem Photobiol. 1996; 63: 123-7.

26. Chacón-Salinas R, Limón-Flores AY, Chávez-Blanco AD, Gonzalez-Estrada A, Ullrich SE. Mast cell-derived IL-10 suppresses germinal center formation by affecting T follicular helper cell function. J Immunol. 2011; 186: 25-31.

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