BIOMARKER ASSESSMENT OF SERUM INTERLEUKIN-18 TOGETHER WITH HOMOCYSTEINE FOR POLYCYSTIC OVARIAN SYNDROME 

Sakar Karem Abdulla a, Ban Mousa Rashid aand Beston Faiek Nore b, c 



a Department of Clinical Biochemistry, College of Pharmacy, University of Sulaimani; Kurdistan Region/ Iraq. 
b Department of Medical Biochemistry, College of Medicine, University of Sulaimani; Kurdistan Region/ Iraq.
c Department of Health, Kurdistan Institution for Strategic Studies and Scientific Research, Sulaimani, Kurdistan Region/ Iraq. 


Submitted: 9/9/2019; Accepted: 6/11/2019; Published: 21/12/2019

ABSTRACT


Background 

Polycystic ovary syndrome (PCOS) a complex endocrine disorder associated with reproductive disorders and metabolic dysfunctions, insulin resistance with compensatory hyperinsulinemia, obesity, endometrial carcinoma, and cardiovascular diseases. 


Objectives

This study aims to evaluate the levels of IL-18 and homocysteine in serum as a possible biomarker for cardiovascular disease in 150 cases positive with PCOS and in 150 negative control females.


Patients and Method

This study was a case-control study and serum samples randomly taken from 300 individuals (150 samples from patients with PCOS and 150 samples from healthy controls). Five milliliters of venous blood has been taken from each individual and the samples were analyzed for interleukin-18 and homocysteine by using enzyme-linked immunosorbent assay, hormones profile include LH, FSH, LH/FSH ratio, TSH, PRL, and Testosterone.


Results

We found that the average level of IL-18 and homocysteine in serum were 378.3 ±181.21 pg/ml and 10.36 ± 5.98 nmol/ml respectively in PCOS patients, while in the control group the values were 224.98± 131.885 pg/ml and 5.17± 5.24 nmol/ml respectively. 


Conclusions

The results show a highly significant difference (p-value < 0.001) and high serum concentration of IL-18 and homocysteine in PCOS as compared to the control group. Therefore, elevation of IL-18 combined with homocysteine is a selective indicator for higher risk of PCOS, which is closely related to cardiovascular abnormality as we have gated for this category of PCOS patients. 



KEYWORDS

Polycystic Ovarian Syndrome, Interleukin-18, Homocysteine, Cardiovascular Disease, Biomarker.