MITOCHONDRIAL ENZYMES CORRELATION WITH BREAST CANCER CLINICOPATHOLOGICAL PARAMETERS/ A STUDY IN SULAIMANI CITY-IRAQ  

Hadeel Adnan Yasseen a, Rawand Tajuldeen Sahib b and Shahow Abdulrehman Ezzaddin C


Department of Pathology, College of Medicine, University of Sulaimani. 
b Ministry of Health.
c Department of Community Medicine, College of Medicine, University of Sulaimani.


Submitted: 30/9/2017; Accepted: 7/2/2017; Published 15/4/2018
ABSTRACT


Background 

Thousands of researches are done for breast cancer, but still cancer epithelial cells relationship with the surrounding stromal cells is a great topic for researchers which is termed the tumor microenvironment.


Objectives 

This study is designed to confirm that presence of the two main mitochondrial enzymes that act in ketone bodies production and utilization and their correlation with the clinicopathological parameters and tumor aggressiveness.


Materials and Methods

This cross sectional study was carried out in Iraq in which 40 selected cases of breast invasive ductal carcinoma not otherwise specified were stained immunohistochemically for two mitochondrial enzymes; HMGCS2 and ACAT1involved in ketone bodies production and utilization respectively and correlate their positivity with clinicopathological parameters of the breast cancer as patient’s age, estrogen and progesterone receptors, HER2, molecular types, tumor grade and lymph node metastasis.


Results

Out of 40 cases, 50% are in the age range of 41-50 years.  Grade I seen in 7%, grade II in 23% and grade III in 70% of cases. Regarding lymph node status, 10% are N2 while 28% are N3. ACAT1 shows stromal significant positivity with N3 only (P. = 0.01). HMGCS2 shows a highly significant epithelial positivity with N2 (P. = 0.004) and a significant stromal positivity with low grade tumors (P. = 0.04). 


Conclusion

No significant correlation between ketone bodies production or utilization with the studied clinicopathological parameters apart from tumor grade and lymph node status, indicating that aggressive and metastatic tumors are more capable of self dependence for feeding themselves with ketone bodies.


KEYWORDS

Ketone bodies, Breast cancer, Mitochondrial enzymes, ACAT1, HMGCS2