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jsmc-10056


MAST CELLS AND APOPTOTIC BODIES IN SEBORRHEIC KERATOSIS: A COMPARATIVE STUDY BETWEEN UVB INDUCED SEBORRHEIC KERATOSIS IN MICE AND SPORADIC CASES IN HUMANS


Nabil AM Salmo *, Azad Kareem Saeed **, Snur Muhammad-Amin Hassan *** and Hasanaein H Khudeir *


*   Department of Pathology and Forensic Pathology, School of Medicine, Sulaimani University.
** Department of Histopathology, College of Veterinary Medicine, Sulaimani University.


Submitted: 17/9/2013; Accepted: 18/2/2014


ABSTRACT

Background 

Exposure to ultraviolet type B (UVB) radiation induces a number of pathologic changes in skin, including erythema, edema, epidermal hyperplasia, sunburn cell formation, immune suppression and eventually leads to cancer development.

Objective

To elucidate the differences in histological appearances of mast cells and apoptotic bodies between the two species (mice and human) among hyperkeratotic and acanthotic types of seborrheic keratosis (SK).

Materials and Methods
Thirty paraffin blocks were used in this study;  fifteen histologically con rmed acanthotic and hyperkeratotic SK cases in human (9 acanthotic and 6 hyperkeratotic) and  fifteen blocks from both types acanthotic and hyperkeratotic SK cases in mice induced by UVB light (9 acanthotic and 6 hyperkeratotic).

Results

Our results revealed that there was a signi cant correlation between mast cells and apoptotic bodies for both groups according to Pearson Correlation test. In human cases mast cells counting ranged between 2-10 with a mean number of 5.2/1HPF, while the total number of apoptotic bodies ranged from 1-4 with a mean number of 2.6/10HPF. When compared to mice cases, the number of mast cells were increased with a range of 12-23 and with a mean number of 19.067/1HPF, while apoptotic bodies were decreased with a range of 3-20/10HPF and with a mean number of 9.4/10HPF.

Conclusion

Dermal mast cells in ltration were remarkably increased in mice skin specimens which were exposed to UVB. The number of apoptotic bodies in UVB induced cases were more than in human sporadic cases.

KEYWORDS

UVB, seborrheic keratosis, apoptotic bodies, mast cells.