ISOLATION AND MOLECULAR IDENTIFICATION OF BRUCELLA MELITENSIS IN ABORTED FETUSES OF SHEEP AND GOATS IN SULAIMANI PROVINCE, IRAQ

Othman Jamal Nassrullah a, Mohammed Omer Mohammad b, and Shwan Kamal Rachid c



a College of Veterinary Medicine, Sulaimani University, Kurdistan Region, Iraq.
b College of the Medicine University of Sulaimani. Kurdistan Region, Iraq.
c University of Charmo, Kurdistan Region, Iraq.


Submitted: 18/12/2019; Accepted: 1/12/2020; Published: 21/12/2020
ABSTRACT


Background 

Brucellosis is a zoonotic disease that is endemic in Sulaimani Province, Iraq. The most accurate, credible, and susceptible method for detecting Brucella DNA is Polymerase Chain Reaction (PCR). A significant increase in abortion rates among the sheep and goats population in Sulaimani Province, Iraq was recorded by Veterinary centers. 


Objectives 

To detect the most common causes of abortion in sheep and goats.


Materials and Methods

A total of 73 aborted fetuses were collected during the delivery season in 2018, 53 sheep and 20 goats. Four sets of primers gene were achieved: firstly, for identification of the positive infection with all possible Brucella genus, the universal ‘catch-all’ primers (OMP2). Others, the specific primers (IS711) for identification Brucella species in all positive and negative samples.


Results

PCR showed that Brucella was the important cause of abortion in sheep and goats (P<0.05). Brucella melitensis was the only species detected, with prevalence rates of (32%) and (60%) in sheep and goats, respectively. Some independent variable risk factors were estimated that were statistically significant, including sex and age of the fetus, age of dam, infected organs of the fetus, history of abortion, and parity. 


Conclusion

There was a significant relationship between abortion and the history of abortion in sheep and goats. Brucella melitensis is a serious reason for abortion in sheep and goats, and a great hazard to public health in Sulaimani Province. 



KEYWORDS

Brucella detection; PCR technique; Isolation, Aborted fetus.